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AIDS VACCINE RESEARCH LABORATORYDavid Watkins, P.I. |
MHC Typing of Rhesus Macaques
University of Wisconsin - National Primate Research Center
In order to have your animals typed, there are three steps.
First, contact the MHC typing core (contact info below) to receive forms needed to set up an account for MHC typing. In addition, we can discuss with you the number of animals that you want typed and what tests you want done to give you an estimate of the cost and turnaround time, and discuss the types of samples available to complete the typing.
| Typing Core Contact info/Shipping Address |
| Gretta Borchardt Rhesus Macaque MHC Typing Core UW AIDS Vaccine Research Lab 555 Science Drive Madison, WI 53711 LAB: (608) 890-0920 FAX: (608) 265-8084 Email: gborchardt@wisc.edu http://ink.primate.wisc.edu/~watkins/services.html |
Second, you will need to set up an account from the University of Wisconsin -National Primate Research Center. Instructions for setting up an account with the WNPRC are as follows (please provide this information to your Purchasing Agent/Contact):
| Billing Accounts |
| Bonnie Gay-Seehawer University of Wisconsin WNPRC 1220 Capitol Ct. Madison, WI 53715 Phone: (608) 890-1173 FAX: (608) 890-1374 Email: bgay@primate.wisc.edu |
A. A purchase order, written in U.S. funds, needs to be issued to: The University of Wisconsin - National Primate Research Center. Generally it is recommended that the purchase order be a blanket or running order which is valid for Center services for a specified period of time. This is due to the fact that a new purchase order and new account will need to be generated if additional services are required after a valid purchase order ends. The order must include address information for submission of invoices, as well as the name, e-mail address, and phone number of the individual who should be contacted with the new account information.
B. The official vendor copy of the purchase order needs to be mailed to or faxed to WNPRC. Faxed purchase orders can only be accepted providing they are complete and legible. We request that the official vendor copy of the purchase order still be mailed to the address above. It is also recommended that you e-mail Bonnie to notify her when you have faxed a purchase order.
C. After the purchase order is received, reviewed and accepted, a WNPRC account will be issued. The contact person specified on the order will then be notified with the new account information.
The WNPRC account must be included when submitting any samples or requests for service. It is the responsibility of the account holder to ensure that no services are requested which will exceed any of the limits in place on their purchase order. In the event that services requested exceed PO limits, data will be held at the lab until WNPRC administration lets us know that your account has had funds added to it.
Please note: Payment is due upon receipt of invoice. Checks must be payable in U.S. funds, drawn on a U.S. bank and issued to The University of Wisconsin - National Primate Research Center. The University of Wisconsin's Tax Identification number is: 39 1805963.
Third, now we are ready to receive your samples.
Preferred sample type(s) (in order of preference for optimal typing results):
1) Blood samples - 2 mL EDTA anticoagulated blood
2) Frozen cells - 2 x 106 nucleated cells, usually in the form of: 2 x 106/1 ml BLCL or 2 x 106/200 ul PBMC
3) DNA - 100uL @ 25ug/mL.
Note: Our PCR method has been optimized for DNA extracted with the Roche MagnaPure system. We achieve optimal typing results from blood or cells extracted on this system and discourage clients from shipping DNA samples extracted by other methods.
Samples should be shipped either on wet ice packs (for blood or DNA) or on dry ice (for frozen cells). FAX (608-265-8084) or email (gborchardt@wisc.edu) the Sample Delivery form when you send the samples. Please also include a copy the Sample Delivery Form with your shipment. When your samples are received, we will let you know they have arrived and will be put in the queue for typing. Our turnaround time is currently about one month, due to the large number of samples that are being typed at any given time. If you wish sequencing confirmation, we will send out a preliminary report containing the PCR-SSP typing, with an estimate of when your sequencing will be finished. Once the sequencing is finished, we will send you a final report.