AIDS VACCINE RESEARCH LABORATORY David Watkins, P.I.

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MHC Typing Rates (as of June 2008, subject to change without notice)
Single allele extraction and typing	$25.00
Single allele, no extraction	$10.00
Tray of 9 SIV restricting MHC alleles	$105.00
For multiple allele requests each sample will be charged $25 for the extraction and genotyping of the first allele, each additional genotyping will be $10.
Sequencing (per allele)#	$75.00
This the maximum sequencing charge per sample and the final charge will depend on the number of positive alleles genotyped.  Samples that are genotyped as negative with only be charged the single allele genotyping fee.  Typically, the number of positive alleles ranges from 0-50% of the alleles typed for in a given sample, depending on the allele frequency.
Rush Charge (per submission)	$50.00
The rush fee will put your samples to the top of the queue for genotyping.  As a courtesy to other researchers, please use this option for critical samples.

NOTE: Clients from private institutions will incur additional fees associated with overhead costs.  Please contact the genotyping core for current pricing.

Refrencing WNPRC Genotyping data:

This typing service is offered with support of NIH grant 5R24RR16038-6.  Our genotyping protocols are published and freely available.  Please cite the appropriate paper(s) below in work describing MHC class I genotyping performed by WNPRC.  Allele frequencies are tracked as a service for all public investigators and may be shared to the research community.  Specific animal data is never redistributed without the investigators consent.

Kaizu M, Borchardt GJ, Glidden CE, Fisk DL, Loffredo JT, Watkins DI, Rehrauer WM. Molecular typing of major histocompatibility complex class I alleles in the Indian rhesus macaque which restrict SIV CD8(+) T cell epitopes. Immunogenetics. 2007 Jul 20; [Epub ahead of print] .

¹Yant LJ, Friedrich TC, Johnson RC, May GE, Maness NJ, Enz AM, Lifson JD, O'Connor DH, Carrington M, Watkins DI. The high-frequency major histocompatibility complex class I allele Mamu-B*17 is associated with control of simian immunodeficiency virus SIVmac239 replication. J Virol. 2006 May;80(10):5074-7. Erratum in: J Virol. 2006 Jul;80(13):6720.

²Loffredo JT, Maxwell J, Qi Y, Glidden CE, Borchardt GJ, Soma T, Bean AT, Beal DR, Wilson NA, Rehrauer WM, Lifson JD, Carrington M, Watkins DI. Mamu-B*08-positive macaques control simian immunodeficiency virus replication. J Virol. 2007 Aug;81(16):8827-32. Epub 2007 May 30.

³Giraldo-Vela JP, Rudersdorf R, Chung C, Qi Y, Wallace LT, Bimber B, Borchardt GJ, Fisk DL, Glidden CE, Loffredo JT, Piaskowski SM, Furlott JR, Morales-Martinez JP, Wilson NA, Rehrauer WM, Lifson JD, Carrington M, Watkins DI. The major histocompatibility complex class II alleles Mamu-DRB1*1003 and -DRB1*0306 are enriched in a cohort of simian immunodeficiency virus-infected rhesus macaque elite controllers. J Virol. 2008 Jan;82(2):859-70.

Disclaimer:

TThe PCR-SSP primers we have developed are based on published sequences of Indian Rhesus MHC Class I or Class II molecules.  We know that there are differences between different populations of animals.  Therefore, depending on your source of animals, allele variants may be falsely detected or not show up on our tests.  We cannot be responsible for these allele variants.  This is the primary reason that we originally sequenced all our positive results, and that we continue to offer this confirmation at an additional charge.