AIDS VACCINE RESEARCH LABORATORY David Watkins, P.I.

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MHC Typing Rates (as of June 2008, subject to change without notice)
Single allele extraction and typing	$25.00
Single allele, no extraction	$10.00
Tray of 9 SIV restricting MHC alleles (Mamu-A*01, A*02, A*08, A*11, B*01, B*03, B*04, B*08, B*17 ) à la carte allele selection	$105.00
For multiple allele requests each sample will be charged $25 for the extraction and genotyping of the first allele, each additional genotyping will be $10.
Sequencing (per allele)#	$75.00
This the maximum sequencing charge per sample and the final charge will depend on the number of positive alleles genotyped. Samples that are genotyped as negative will only be charged the single allele genotyping fee. Typically, the number of positive alleles ranges from 0-50% of the alleles typed for in a given sample, depending on the allele frequency.
Rush Charge (per 25 samples)	$50.00
The rush fee will put your samples to the top of the queue for genotyping. As a courtesy to other researchers, please use this option only for critical samples.

NOTE: We are permitted to offer this service at cost (labor and consumables) to academic researchers. We do not want to exclude private researchers, but because our funding is from public monies, we are mandated to include overhead costs in pricing for these investigators.  Please contact the genotyping core for current industry pricing.

Refrencing WNPRC Genotyping data:

This typing service is offered with support of NIH grant 5R24RR016038.  Our genotyping protocols are published and freely available.  Please cite the appropriate paper(s) below in work describing MHC class I genotyping performed by the University of Wisconsin.  Allele frequencies are tracked as a service for investigators and may be shared with the research community.  Specific animal data is never redistributed without the investigators consent.

Kaizu M, Borchardt GJ, Glidden CE, Fisk DL, Loffredo JT, Watkins DI, Rehrauer WM. Molecular typing of major histocompatibility complex class I alleles in the Indian rhesus macaque which restrict SIV CD8(+) T cell epitopes. Immunogenetics. 2007 Jul 20; [Epub ahead of print] .

¹Yant LJ, Friedrich TC, Johnson RC, May GE, Maness NJ, Enz AM, Lifson JD, O'Connor DH, Carrington M, Watkins DI. The high-frequency major histocompatibility complex class I allele Mamu-B*17 is associated with control of simian immunodeficiency virus SIVmac239 replication. J Virol. 2006 May;80(10):5074-7. Erratum in: J Virol. 2006 Jul;80(13):6720.

²Loffredo JT, Maxwell J, Qi Y, Glidden CE, Borchardt GJ, Soma T, Bean AT, Beal DR, Wilson NA, Rehrauer WM, Lifson JD, Carrington M, Watkins DI. Mamu-B*08-positive macaques control simian immunodeficiency virus replication. J Virol. 2007 Aug;81(16):8827-32. Epub 2007 May 30.

³Giraldo-Vela JP, Rudersdorf R, Chung C, Qi Y, Wallace LT, Bimber B, Borchardt GJ, Fisk DL, Glidden CE, Loffredo JT, Piaskowski SM, Furlott JR, Morales-Martinez JP, Wilson NA, Rehrauer WM, Lifson JD, Carrington M, Watkins DI. The major histocompatibility complex class II alleles Mamu-DRB1*1003 and -DRB1*0306 are enriched in a cohort of simian immunodeficiency virus-infected rhesus macaque elite controllers. J Virol. 2008 Jan;82(2):859-70.