Hormone Assays
N ATIONAL P RIMATE R ESEARCH C ENTER

ASSAY SERVICES UNIT

Assay Services provides up-to-date, efficient and cost-effective hormone measurements in a centralized facility for the Primate Center's core staff, affiliated scientists and the primate community at large. Our versatile approach helps investigators find effective and affordable solutions to a host of endocrine challenges. You can request assays or assay development from our service at any time by following our First 5 Steps in Requesting Assay Service or by contacting Toni Ziegler, Ph.D. Our current listing of endocrine procedures and hormone assays will give you up-to-date information on available services.

An additional part of Assay Services' mission involves continuing developments of new methodology and techniques to meet the progressive research requirements of investigators. These developments include non-invasive, non-isotopic assays, and endocrine analyses from fecal samples that enable investigators to achieve stress-free sampling from animals in captivity. We also have well-established techniques to monitor the endocrinology of reproduction and the reproductive status of primates in the wild. Our expansion of assays for metabolic and bone physiology biomarkers is proving especially useful for studying aging in primates.

The following items provide useful information regarding assay services and services available to investigators:

The First 5 Steps in Requesting Assay Service
Sample handling and preparation
Our current hormone assays and procedures
An introduction and some background history to Assay Services
Quality control
Information on our next lab meeting

Key information regarding the different formats, sample materials and procedures that are available to investigators:

Techniques Media Old World Primates & Apes New World Primates & Prosimians
RIA CSF Macaca mulatta Callithrix jacchus
IRMA Blood Macaca fuscata Saguinus oedipus
EIA Urine Macaca silenus Brachyteles arachnoides
ECL Feces Macaca fascicularis Cebus apella
HPLC-UV Cell culture Cercopithecus mitis Cebuella pygmaea
Bioassay Follicular fluid Theropithecus gelada Lagothrix lagotricha
Celite chromatography Oviductal fluid Erythrocebus patas Callithrix kuhli
Iodination Tissue extract Nasalis larvatus Saimiri sciureus
Protein determination Saliva Pongo pygmaeus Alouatta caraya
LC/MS Hair Gorilla gorilla Microcebus murinus
HPLC-ECD Pituitary Extract Homo sapiens Lemur catta
Solid Phase Extraction   Procolobus badius Cebus capucinus
    Cercopithecus aethiops Varecia variegata variegata
    Papio sp. Eulemur rubriventer
      Alouatta palliata
      Callithrix geoffroyi
      Callimico goeldii

For more information, contact Toni Ziegler, Ph.D.

©2003 University of Wisconsin System Board of Regents
Maintained by Fritz Wegner
Last updated: Monday, October 8th, 2007

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