Quality Controls

HPLC based QC

  • Blanks
  • Recovery
  • Linerarity
  • LLOQ (lower limits of quantification)
  • LLOD (lower limits of detection)
  • Intra and inter assay coefficient of variation (CV)

Immunoassay based QC

  • Intra and inter assay coefficient of variation (CV)
  • Parallelism
  • Accuracy
  • Sensitivity of the assay

Examples of HPLC based QCs

25-hydroxyvitamin D2 QC Summary for Marmoset Serum samples
LLOD = 0.5 ng/ml, r=0.9999 for calibration curve, blanks = 0
QC Pool IDUTAK Verified ValueUTAK Expected Range WNPRC Mean Value MRM1/MRM2 (n=3)WNPRC Mean % Recovery MRM1/MRM2 Std. Dev. MRM1/MRM2CV MRM1/MRM2
UTAK Low Control119-138.4/8.5 76.5/77.00.3/0.73.1/8.0
UTAK Medium Control3328-3826.3/26.1 79.7/79.01.9/1.77.1/6.5
UTAK High Control7765-8965.4/64.1 85.0/83.32.4/3.23.7/4.9

Examples of Immunoassay based QCs

*PdG = pregnanediol-glucuronide
QC pool IDMediaAssay typeParallelism AccuracySensitivityIntra assay CV %Inter assay CV %
MarmosetserumInsulin RIAt=-1.67, df=26, P > 0.05 98.89+2.38 s.e.m.0.25 µU/ml2.874.89
Low Rhesusurine*PdG EIAt=-1.77, df=23, P > 0.05 104.01+1.41 s.e.m.9 pg4.413.9
High RhesusurinePdG EIAt=-1.77, df=23, P > 0.05 104.01+1.41 s.e.m.9 pg3.011.6